In vitro analysis of the stimulation of bone formation by highly bioactive apatite- and wollastonite-containing glass-ceramic: Released calcium ions promote osteogenic differentiation in osteoblastic ROS17/2.8 cells

Author(s):  
H. Matsuoka ◽  
H. Akiyama ◽  
Y. Okada ◽  
H. Ito ◽  
C. Shigeno ◽  
...  
2019 ◽  
Vol 22 (1) ◽  
pp. Process
Author(s):  
Karen Cristina Archangelo ◽  
Marcela Moreira Penteado ◽  
Amanda Maria de Oliveira Dal Piva ◽  
Andrea Souza Nogueira ◽  
João Paulo Mendes Tribst ◽  
...  

This short report evaluated the differences in stress concentration and the load to fracture of multilayered and monolayer glass ceramic discs. Using a static structural analysis, the 3D model of the discs received a load of 150N and results in maximum principal stress were obtained. For the in vitro analysis, the samples (ø 12 mm) were submitted to a compressive test (100kgf, 1mm/min). The data was analyzed using one-way analysis of variance and Tukey test (α=5%). The monolayer group showed a lower stress peak (129.24 MPa) and higher load to fracture (118.38N) than the multilayered group with 211.04MPa and 48.34N, respectively. All samples presented catastrophic failure with its origin on the tensile surface. Therefore, the monolayer ceramic group showed superior mechanical behavior than the multilayered group.


2019 ◽  
Vol 5 (2) ◽  
pp. 23-27
Author(s):  
K Premnath ◽  
◽  
Nagaranjani Prakash ◽  
Mohammed Ajmal B ◽  
Syed Ahmed Raheel ◽  
...  

Author(s):  
R.A. Milligan ◽  
P.N.T. Unwin

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.


2005 ◽  
Vol 173 (4S) ◽  
pp. 315-316
Author(s):  
Kari Hendlin ◽  
Brynn Lund ◽  
Manoj Monga

1999 ◽  
Vol 81 (06) ◽  
pp. 951-956 ◽  
Author(s):  
J. Corral ◽  
R. González-Conejero ◽  
J. Rivera ◽  
F. Ortuño ◽  
P. Aparicio ◽  
...  

SummaryThe variability of the platelet GP Ia/IIa density has been associated with the 807 C/T polymorphism (Phe 224) of the GP Ia gene in American Caucasian population. We have investigated the genotype and allelic frequencies of this polymorphism in Spanish Caucasians. The T allele was found in 35% of the 284 blood donors analyzed. We confirmed in 159 healthy subjects a significant association between the 807 C/T polymorphism and the platelet GP Ia density. The T allele correlated with high number of GP Ia molecules on platelet surface. In addition, we observed a similar association of this polymorphism with the expression of this protein in other blood cell types. The platelet responsiveness to collagen was determined by “in vitro” analysis of the platelet activation and aggregation response. We found no significant differences in these functional platelet parameters according to the 807 C/T genotype. Finally, results from 3 case/control studies involving 302 consecutive patients (101 with coronary heart disease, 104 with cerebrovascular disease and 97 with deep venous thrombosis) determined that the 807 C/T polymorphism of the GP Ia gene does not represent a risk factor for arterial or venous thrombosis.


2018 ◽  
Vol 18 ◽  
Author(s):  
Chaitra Venugopal ◽  
Christopher Shamir ◽  
Sivapriya Senthilkumar ◽  
Janitri Venkatachala Babu ◽  
Peedikayil Kurien Sonu ◽  
...  

Author(s):  
SHREYASHI M ◽  
SULAGNA D ◽  
SANKARI D ◽  
THIRUMURUGAN D ◽  
INFANT SANTHOSE B ◽  
...  

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